#!/bin/sh # # Downloads sequence for the GRCm38 release 81 version of M. Musculus (mouse) from # Ensembl. # # By default, this script builds and index for just the base files, # since alignments to those sequences are the most useful. To change # which categories are built by this script, edit the CHRS_TO_INDEX # variable below. # ENSEMBL_RELEASE=84 ENSEMBL_GRCm38_BASE=ftp://ftp.ensembl.org/pub/release-${ENSEMBL_RELEASE}/fasta/mus_musculus/dna get() { file=$1 if ! wget --version >/dev/null 2>/dev/null ; then if ! curl --version >/dev/null 2>/dev/null ; then echo "Please install wget or curl somewhere in your PATH" exit 1 fi curl -o `basename $1` $1 return $? else wget $1 return $? fi } HISAT2_BUILD_EXE=./hisat2-build if [ ! -x "$HISAT2_BUILD_EXE" ] ; then if ! which hisat2-build ; then echo "Could not find hisat2-build in current directory or in PATH" exit 1 else HISAT2_BUILD_EXE=`which hisat2-build` fi fi rm -f genome.fa F=Mus_musculus.GRCm38.dna.primary_assembly.fa if [ ! -f $F ] ; then get ${ENSEMBL_GRCm38_BASE}/$F.gz || (echo "Error getting $F" && exit 1) gunzip $F.gz || (echo "Error unzipping $F" && exit 1) mv $F genome.fa fi CMD="${HISAT2_BUILD_EXE} genome.fa genome" echo Running $CMD if $CMD ; then echo "genome index built; you may remove fasta files" else echo "Index building failed; see error message" fi