#!/bin/sh # # Downloads sequence for the rn6 version of R. norvegicus (rat) from # UCSC. # # Note that UCSC's rn6 build has two categories of compressed fasta # files: # # 1. The base files, named chr??.fa.gz # 2. The unplaced-sequence files, named chr??_random.fa.gz # # By default, this script indexes all these files. To change which # categories are built by this script, edit the CHRS_TO_INDEX # variable below. # RN6_BASE=ftp://hgdownload.cse.ucsc.edu/goldenPath/rn6/bigZips F=rn6.fa.gz get() { file=$1 if ! wget --version >/dev/null 2>/dev/null ; then if ! curl --version >/dev/null 2>/dev/null ; then echo "Please install wget or curl somewhere in your PATH" exit 1 fi curl -o `basename $1` $1 return $? else wget $1 return $? fi } HISAT2_BUILD_EXE=./hisat2-build if [ ! -x "$HISAT2_BUILD_EXE" ] ; then if ! which hisat2-build ; then echo "Could not find hisat2-build in current directory or in PATH" exit 1 else HISAT2_BUILD_EXE=`which hisat2-build` fi fi rm -f genome.fa get ${RN6_BASE}/$F || (echo "Error getting $F" && exit 1) gzip -cd $F > genome.fa || (echo "Error unzipping $F" && exit 1) rm $F CMD="${HISAT2_BUILD_EXE} -p 4 genome.fa genome" echo Running $CMD if $CMD ; then echo "genome index built; you may remove fasta files" else echo "Index building failed; see error message" fi