#!/bin/sh # # Downloads sequence for A. thaliana from TAIR v10 and build Bowtie 2 index. # GENOMES_MIRROR=ftp://ftp.arabidopsis.org/home/tair get() { file=$1 if ! wget --version >/dev/null 2>/dev/null ; then if ! curl --version >/dev/null 2>/dev/null ; then echo "Please install wget or curl somewhere in your PATH" exit 1 fi curl -o `basename $1` $1 return $? else wget $1 return $? fi } BOWTIE_BUILD_EXE=./bowtie2-build if [ ! -x "$BOWTIE_BUILD_EXE" ] ; then if ! which bowtie2-build ; then echo "Could not find bowtie2-build in current directory or in PATH" exit 1 else BOWTIE_BUILD_EXE=`which bowtie2-build` fi fi FC= for c in 1 2 3 4 5 C M ; do if [ ! -f TAIR10_chr$c.fas ] ; then FN=TAIR10_chr$c.fas F=${GENOMES_MIRROR}/Sequences/whole_chromosomes/${FN} [ -n "$FC" ] && FC="$FC,$FN" [ -z "$FC" ] && FC=$FN get $F || (echo "Error getting $F" && exit 1) fi if [ ! -f TAIR10_chr$c.fas ] ; then echo "Could not find chr$c.fas file!" exit 2 fi done CMD="${BOWTIE_BUILD_EXE} $* $FC a_thaliana" echo $CMD if $CMD ; then echo "a_thaliana index built; you may remove fasta files" else echo "Index building failed; see error message" fi